Enzyme kinetics and assay design
Select a preparation and compatible measurement method. Plan substrate series, blanks and suitable observation intervals before drawing conclusions from rate measurements.
View the enzyme at S&T ↗Enzymes for university teaching laboratories.
Build undergraduate practicals and student projects around a defined biochemical question. Individual enzymes and complementary collections support investigations in enzyme kinetics, analytical readouts, substrate specificity and applied biotechnology.
Explore the enzyme collection ↗Enzyme experiment ideas
β-Glucosidase and glucose release
Investigate cellobiose conversion or complementary action with cellulase using a compatible assay and controls for initial glucose content.
View the enzyme at S&T ↗Glucose oxidase and coupled assays
Explore enzyme-based sensing with a validated coupled assay. Supporting reagents and the specificity of the measured signal are part of the experimental design.
View the enzyme at S&T ↗Laccase and oxidative biocatalysis
Use an appropriate chromogenic substrate and validated conditions to study oxidative enzyme action. Substrate and assay selection determine the useful readout.
View the enzyme at S&T ↗Plan a meaningful practical
Specify the assay conditions and activity units for the preparation in use. Check background signals, linear response, substrate suitability and the effect of dilution. Commercial preparations may contain stabilisers or other components relevant to analytical interpretation. Confirm technical details before building a teaching protocol.
Choose kits and supplies for your teaching lab
Choose individual packs for one practical or a ProStar enzyme collection for a broader teaching programme. Discuss cohort size, repeat teaching requirements, documentation, pack sizes and remaining shelf life with S&T. The calculator separates allocated experiment costs from whole-pack purchase costs.
Investigation ideas, not a complete practical protocol. Check product-specific instructions, SDS, assay compatibility and your institution’s assessment before use.